Table 3. Ad5-Δ24RGD viral replication in doxorubicin-treated OS cells as determined by quantitative PCR analysis.
| OS cells | Genomes × 105 −doxa | genomes × 105 +dox | +dox/−dox | Average ratio: +dox/−dox (±s.d.) |
|---|---|---|---|---|
| 5.8 | 3.5 | 0.6 | ||
| OS-11 | 3.3 | 0.9 | 0.3 | 0.4 (±0.2) |
| 4.1 | 1.1 | 0.3 | ||
| 6.5 | 1.4 | 0.2 | ||
| OS-12A | 1 | 0.2 | 0.2 | 0.2 (±0.04) |
| 3.4 | 0.5 | 0.1 | ||
| 17 | 16 | 0.9 | ||
| OS-16 | 8.9 | 14 | 1.6 | 1.3 (±0.3) |
| 75 | 94 | 1.3 | ||
| 2.1 | 2.9 | 1.4 | ||
| SaOs-2 | 5.5 | 6.4 | 1.2 | 1.1 (±0.3) |
| 11 | 8 | 0.7 | ||
| 1.6 | 3.4 | 2.1 | ||
| MG-63 | 7.7 | 7 | 0.9 | 1.2 (±0.8) |
| 11 | 6.2 | 0.6 | ||
| 20 | 15 | 0.8 | ||
| U2OS | 96 | 127 | 1.3 | 1.0 (±0.3) |
| 87 | 72 | 0.8 | ||
| 2.1 | 4.8 | 2.3 | ||
| SaOs-2b | 5.5 | 5.1 | 0.9 | 1.8 (±0.8) |
| 11 | 25 | 2.3 |
a
The number of adenovirus genomes in cells cultured in the absence (−dox) or presence (+dox) of a subtoxic dose doxorubicin was determined in three independent experiments and the average ratio and s.d. was calculated.
b
SaOs-2 cells treated with a toxic dose of doxorubicin.
PCR=polymerase chain reaction; OS=osteosarcoma.