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. 2006 Feb 14;94(6):914–921. doi: 10.1038/sj.bjc.6602984

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Copyright © 2006 Cancer Research UK

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Bisulphite-sequencing analysis of TCF2 in ovarian cancer cell lines. (A) PCR products used for COBRA analysis were electrophoresed in agarose, excised, purified and directly sequenced. In normal ovary and TOV112D and MCAS cells, all of the cytosines within the CpG sites were substituted with thymines. In AMOC2, KFr, Caov-3 and HTBOA cells, all cytosines in the CpG sites remained cytosines. In PAI-1 cells, the CpG sites contained a mixture of cytosines and thymines, as methylation was partial. U: unmethylated CpG sites; M: methylated CpG sites. (B) Summary of bisulphite-sequencing. Unmethylated CpG sites are indicated by open circles, and methylated CpG sites are indicated by filled circles. CpG sites that show a mixed pattern of methylated and unmethylated alleles are shown in the grey circles. Cell lines are indicated on the left. The CpG sites in the region analysed are indicated by vertical bars (top). The CpG site examined by COBRA analysis is indicated as TaiI.