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. Author manuscript; available in PMC: 2008 Apr 30.
Published in final edited form as: J Biol Chem. 2007 Jul 30;282(38):28149–28156. doi: 10.1074/jbc.M703409200

FIGURE 1. ERAD of ENaC α subunit in yeast requires sHsps for maximal efficiency.

FIGURE 1

ENaC HAαV5 degradation was measured by lysing cells at various times during a chase following cycloheximide addition, and protein levels were quantified by Western analysis and normalized to Sec61 levels. ENaC α subunit degradation was measured for both wild type (○) and mutant (●) yeast, as indicated. n = 6 (hsp26Δhsp42Δ) or 4 (ufd1-1, cim3-1). *, p < 0.05 versus wild type yeast, determined by Student's t test.