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. Author manuscript; available in PMC: 2008 Apr 30.
Published in final edited form as: J Biol Chem. 2007 Aug 14;282(42):30618–30628. doi: 10.1074/jbc.M701840200

FIGURE 3. Sbh1p and Sbh2p are dispensable for pΔgpαf ERAD.

FIGURE 3

A, mutant α-factor precursor (pΔgpαf) was translocated into wild type or mutant microsomes, membranes were washed, and ERAD was initiated by adding yeast cytosol ATP and an ATP-regenerating system, and incubation at 30 °C. At the indicated time points, samples were precipitated with trichloroacetic acid and proteins analyzed by SDS-PAGE and phosphorimaging. The asterisk indicates untranslocated pΔgpαf associated with the cytosolic face of the microsomes. B, quantitation of Δgpαf on the gels shown in A. △, SBH1 SBH2; ♦, sbh1Δ sbh2Δ.