Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Jan 15;98(2):345–355. doi: 10.1038/sj.bjc.6604160

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2008 Cancer Research UK

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Apoptosis was assessed by TUNEL assay. The TUNEL assay was used for the morphological detection of any apoptotic changes. U251MG or D54MG cells with siRNA/control or survivin transfection, nonirradiated or irradiated at a dose of 4 Gy, were cultured for 3 (72 h) or 5 days (120 h), and were stained according to the manufacturer's instructions after fixation with 4% formaldehyde. The AI was defined as the percentage of TUNEL-positive U251 (A) or D54MG cells (B). (C) TUNEL signals were detected by fluorescence microscopy in the nuclei of some irradiated and siRNA/control-transfected U251MG cells.