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. 2008 Jan 22;98(2):410–417. doi: 10.1038/sj.bjc.6604124

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Copyright © 2008 Cancer Research UK

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Biologic characteristics of GNG7-transfected oesophageal cancer cells. (A) Invasion assay for GNG7 transfectants. After incubation for 6 or 24 h, the number of cells on the lower surface of the membrane was counted in five different fields (× 200). Clone A and clone B GNG7 transfectants were compared with the parental cell line KY150 and the mock cell line. GNG7 transfectants A (average intensity=2.08) and B (average intensity=1.87) were significantly less invasive than parental cells (average intensity=2.55) and mock cells (average intensity=2.82) (Figure 3, P<0.05). (B) Reactivation of GNG7 expression by 5-AZAC treatment. Analysis of four representative oesophageal cancer cell lines. Data indicate that GNG7 expression may be regulated by methylation. (C) Increased expression of GNG7 after 5-AZAC treatment in 15 oesophageal cancer cell lines. The vertical axis indicates the calculated ratio of GNG7 expression with and without 5-AZAC treatment.