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. 2005 Jun 28;93(2):248–259. doi: 10.1038/sj.bjc.6602679

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Copyright © 2005 Cancer Research UK

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Relative HPV16-specific antibody levels obtained using an ELISA that employed HPV16 L1-VLPs. Antibody levels were quantitated by relating the absorbencies obtained for each sample to that obtained from the positive control (Camvir 1) run under standard conditions on every assay plate. The negative control providing the negative cutoff value (—) was taken as 2 s.d. above the mean of the absorbencies obtained using the virgin controls. Background readings, where no antigen had been bound to the plate, were subtracted from experimental readings in every case.