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. 2005 Jul 5;93(2):224–232. doi: 10.1038/sj.bjc.6602685

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Copyright © 2005 Cancer Research UK

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Lewis lung carcinoma cells were cultured under normal growth conditions and proteins were isolated from the cell line and from lysed murine anaemic spleens. Following separation of proteins by SDS–PAGE, proteins were transferred to a nitrocellulose membrane and probed with anti-EpoR antibody. EpoR expression was analysed by X-ray autoradiography. Data represent typical results of one of three independent experiments.