Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Jul 12;93(3):310–318. doi: 10.1038/sj.bjc.6602700

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005 Cancer Research UK

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Liganded RARβ2 modulates CYP26A1 expression. RARβ2 gene expression was analysed with the standard competitive RT–PCR, and CYP26A1 expression was analysed by competitive RT–PCR in RA-sensitive SH-SY5Y, Calu-6, and T47D cells and noncompetitive RT–PCR in RA-resistant cells, with housekeeping gene β2M as an internal control. (A, B) CYP26A1 transcription was determined in MDA-MB-231 (A) or SK-MES-1 cells (B) transiently transfected with RARβ2 cDNA plasmid or vector plasmid and treated with control or 10 μM aRA for 24 h (Lanes 2 and 5) or 3 days (lanes 3 and 6). Last lane in (B) contained the positive control from Calu-6 cells treated with 10 μM aRA for 3 days. (C) RARβ2 and CYP26A1 expression was analysed in SH-SY5Y, Calu-6, and T-47D cells transfected with scramble siRNA or RARβ2 siRNA and treated with 10 μM aRA for 48 h.