Table 2. Comparison of target gene fold induction by aRA between microarray data (outside parenthesis) and competitive RT–PCR results (inside parenthesis) in neuroblastoma cells treated with 10 μM aRA.
|
SH-SY5Y
|
BE(2)-C
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Gene description | GeneBank accession | 1 h | 24 h | 3 days | 7 days | 1 h | 24 h | 3 days | 7 days |
| RGS16 | AA453774 | 2.0 (2.4) | 4.0 (4.1) | 3.0 (2.9) | 2.2 (1.9) | 1.7 (1.9) | 2.9 (3.2) | 1.9 (2.6) | 1.7 (1.9) |
| TIA1 | N59426 | 0.2 (0.9) | 0.1 (0.8) | 0.2 (0.9) | 0.2 (0.9) | 0.6 (1.2) | 0.6 (0.7) | 0.6 (0.8) | 0.5 (0.6) |
| RARβ2 | AA419238 | 0.9 | 0.9 | 1.5 | 2.6 | 3.1 | 4.0 | 4.0 | 5.4 |
| CYP26A1 | R51021 | 1.1 (—) | 11 (91) | 11 (100) | 17 (94) | 0.8 (—) | 1.5 (36) | 3.3 (87) | 7.3 (94) |
| CRBP1 | AA700832 | 1.2 (1.0) | 2.2 (1.9) | 3.0 (1.7) | 3.2 (1.6) | 0.8 (1.2) | 2.0 (1.6) | 2.9 (2.8) | 3.6 (2.3) |
| CRABP1 | AA454702 | 1.1 | 2.6 | 2.4 | 4.3 | 1.0 | 1.2 | 2.2 | 5.1 |
| ATP7A | AA236141 | 1.0 (1.7) | 2.0 (2.6) | 4.5 (4.2) | 7.2 (3.9) | 1.0 (1.1) | 2.1 (4.6) | 3.9 (16) | 6.3 (23) |
| IL13RA2 | R52796 | 1.1 | 1.2 | 1.7 | 5.1 | 1.0 | 2.0 | 2.0 | 3.2 |
| DUSP6 | AA630374 | 1.2 | 3.0 | 5.5 | 5.5 | 0.8 | 3.5 | 6.7 | 5.9 |
| EGR1 | AA486533 | 1.0 (1.0) | 2.4 (3.4) | 2.6 (1.3) | 3.4 (1.3) | 1.4 (0.3) | 2.6 (1.2) | 2.0 (1.3) | 1.9 (1.5) |
| RET | H24956 | 1.2 (0.9) | 3.3 (2.7) | 4.0 (2.3) | 4.0 (1.8) | 1.2 (0.7) | 2.5 (1.4) | 2.9 (2.7) | 2.3 (2.1) |
| PLAT | AA447797 | 0.9 (1.4) | 3.1 (1.5) | 3.3 (1.8) | 17 (2.0) | 1.0 (1.4) | 2.0 (1.9) | 3.9 (2.3) | 6.1 (2.4) |
| FLNB | AA486239 | 1.1 (1.2) | 2.1 (5.2) | 3.2 (4.2) | 8.5 (6.0) | 1.2 (0.7) | 3.7 (2.0) | 4.3 (2.5) | 10 (3.2) |
| ITGA1 | H68922 | 1.1 | 2.4 | 3.4 | 6.5 | 0.9 | 2.4 | 3.5 | 3.7 |
| TMSB4X | AA634103 | 1.3 | 2.3 | 3.7 | 8.3 | 1.0 | 1.4 | 3.4 | 4.5 |
| HOXD9 | AA424871 | 1.2 | 2.0 | 2.5 | 3.4 | 1.1 | 1.5 | 1.7 | 2.0 |
| CNN2 | AA284856 | 1.2 | 2.2 | 1.9 | 3.5 | 0.9 | 1.0 | 1.6 | 2.1 |
| TRB2 | AA458653 | 1.3 | 2.2 | 4.0 | 6.3 | 1.3 | 1.8 | 2.7 | 3.4 |
| CXCR4 | AA621824 | 1.1 | 0.5 | 0.4 | 0.4 | 1.0 | 1.0 | 0.5 | 0.5 |
| TM4SF3 | AA045699 | 1.1 | 1.3 | 2.8 | 3.8 | 1.2 | 1.1 | 2.7 | 6.0 |
| DCX | AA620421 | 1.0 | 1.1 | 2.9 | 3.1 | 1.1 | 1.3 | 3.8 | 3.4 |
| ASL | AA486741 | 1.1 | 1.9 | 3.0 | 3.2 | 1.1 | 1.8 | 2.9 | 2.5 |
| DCN | AA099394 | 1.1 | 1.3 | 2.6 | 2.5 | 1.1 | 1.1 | 1.9 | 2.8 |
| TP53I3 | AA668595 | 1.1 | 1.1 | 1.6 | 3.0 | 1.2 | 1.4 | 2.9 | 5.1 |
| IGFBP3 | AA598601 | 1.1 | 0.5 | 0.8 | 3.2 | 0.9 | 0.9 | 2.4 | 4.6 |
| AF1Q | AA456008 | 1.3 | 1.7 | 2.8 | 3.4 | 1.2 | 1.5 | 1.9 | 2.8 |
| DLK1 | AA576129 | 1.5 (0.9) | 0.6 (0.7) | 0.5 (0.6) | 0.2 (0.4) | 1.0 (1.1) | 1.2 (1.0) | 0.3 (0.7) | 0.1 (0.5) |
| SGNE1 | AA670429 | 1.1 | 1.1 | 1.5 | 3.1 | 0.9 | 1.0 | 1.7 | 2.7 |
| PTN | AA001449 | 1.2 | 1.0 | 1.5 | 2.9 | 1.0 | 1.2 | 1.8 | 2.5 |
| SMAD3 | W72201 | 1.1 (1.2) | 1.2 (2.7) | 1.8 (2.1) | 3.1 (2.1) | 0.9 (1.1) | 1.4 (1.4) | 1.6 (1.7) | 3.0 (2.7) |
| PTPRU | AA644448 | 1.2 | 0.7 | 0.6 | 0.5 | 1.2 | 0.7 | 0.6 | 0.4 |
(—) represents undetectable by PCR.