Table 2. Density of ED1 and ED2 macrophages and CD4 and CD8 lymphocytes in implanted tumour in treated rats (n=8 in each group).
|
ED1-positive
macrophagesa
|
ED2-positive
macrophagesa
|
CD4 lymphocytesa
|
CD8 lymphocytesa
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| Group | Implantation post-treatment, weeks/killing, days after challenge | Tumour capsule | Viable tumour | Tumor capsule | Viable tumour | Tumour capsule | Viable tumour | Tumour capsule | Viable tumour |
| I. Interstitial laser thermotherapy (ILT) | |||||||||
| 2w/6d | 76±25 | 74±24 | 0.2±0.4 | 0.8±1.0 | 0.8±0.8 | 0±0 | 14±8.3* | 15±7.6* | |
| 5w/6d | 58±14 | 54±19 | 0±0 | 0.6±0.5 | 0±0 | 0±0 | 27±16* | 20±22* | |
| 10w/6d | 65±29 | 79±13 | 0.5±0.5 | 0.5±0.5 | — | 0.3±0.5 | 17±23* | 9.8±6.1* | |
| 2w/12d | 76±35* | 64±29* | 0.4±0.5 | — | 0.5±0.9 | 0±0 | 29±24* | 11±15* | |
| 5w/12d | 73±19* | 73±31* | 0±0 | 0.6±1.0 | 0.1±0.3 | 0±0 | 20±12* | 18±15* | |
| 10w/12d | 62±15 | 65±22 | 1.1±1.1 | 0.8±1.2 | 1.4±1.3 | 0.2±0.4 | 12±13 | 26±10 | |
| 2w/48db | 19±23 | — | 0.3±0.6 | 0.5±0.7 | — | — | 3.3±4.2 | 0.5±0.7 | |
| 5w/48d | 46±26 | — | 0±0 | — | 0±0 | — | 1.1±1.2 | — | |
| 10w/48d | 37±12 | — | 1.0±1.4 | — | 1.0±1.4 | — | 2.5±2.1 | — | |
| II. Resection of tumour-bearing liver lobe | |||||||||
| 2w/6d | 77±20 | 73±18 | 0.3±0.8 | 0.1±0.4 | 0.3±0.5 | 0.7±0.8 | 13±14 | 10±5.2 | |
| 5w/6d | 63±13 | 62±12 | 1.2±1.6 | 1.5±0.8 | 0±0 | 0±0 | 17±16 | 2.3±3.4 | |
| 10w/6d | 91±20 | 89±29 | 0.25±0.5 | 1.0±1.4 | 0.5±0.6 | 0.6±0.5 | 11±5.9 | 14±7.8 | |
| 2w/12d | 64±25 | 54±29 | 0.9±1.6 | 0.4±0.5 | 0±0 | 0±0 | 14±12 | 12±8.6 | |
| 5w/12d | 58±14 | 49±13 | 0.3±0.5 | 0.2±0.4 | 0.2±0.4 | 0.2±0.4 | 17±8.4 | 11±10 | |
| 10w/12d | — | — | — | — | — | — | — | — | |
| 2w/48dc | — | — | — | — | — | — | — | — | |
| 5w/48dc | 25±21 | 0 | 1.0±1.7 | 1.0±1.4 | 1.0±1.4 | 1.0±1.4 | 1.3±1.1 | 0±0 | |
| 10w/48dc | — | — | — | — | — | — | — | — | |
w=weeks; d=days.
a
Number of cells per visual field.
b
Two rats with signs of vital tumour in the liver.
c
Animals had to be euthanised 10–30 days after reimplantation and only two tumours were analysed. Values are means±s.e.m.
*
P<0.05 for comparisons between ILT and resection of tumour-bearing liver lobe. For comparison between treatments, values were pooled for each post-treatment challenge day, since there were no differences within each day for different weeks after treatment.