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. 2005 Aug 9;93(4):478–482. doi: 10.1038/sj.bjc.6602726

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Copyright © 2005 Cancer Research UK

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Construction of Checkerboard TMA from prostatic needle biopsies. (A) Prostatic needle biopsies embedded horizontally within a paraffin block (bottom) and H&E sections from this block (top). (B) Checkers (4 × 2 × 2 mm³) cut from the original paraffin block containing the prostatic needle biopsy specimen was painted on the surface opposite that containing the biopsy, alternately with blue and red dyes. (C) Checkers positioned in a 36 × 24 × 5 mm³ stainless steel mould (Raymond A Lamb Ltd, UK) so that each biopsy specimen now had a vertical orientation. (D) Finished Checkerboard TMA block in which the cross-sections of the vertically positioned biopsies are seen (arrow). (E) Haematoxylin and eosin-stained section (4 μm) obtained from the Checkerboard TMA block.