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. 2005 Aug 2;93(4):458–463. doi: 10.1038/sj.bjc.6602716

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Copyright © 2005 Cancer Research UK

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Expression of TSPY mRNA in normal and malignant tissues. Testis-specific protein Y-encoded mRNA expression was analysed by RT–PCR. (A) TSPY mRNA was expressed only in normal testis but not in other tissues. Lane 1: brain; 2: heart; 3: kidney; 4: liver; 5: lung; 6: pancreas; 7: skeletal muscle; 8: placenta; 9: ovary; 10: thymus; 11: prostate; 12: spleen; 13: testis; 14: small intestine; 15: colon; 16: leucocytes; 17: negative control; 18: positive control (testis as a positive control). (B) Expression of TSPY mRNA in HCC tissues. Representative expression pattern is shown in some HCC samples; testis mRNA was used as a positive control. RT–PCR for G3PDH was used to monitor the quality of the RNA sample. (T: cancerous tissues; N: adjacent noncancerous tissues; P: positive control). (C) Expression of TSPY mRNA in HCC cell lines. Lane 1: hep-hcc-1; lane 2: hep-hcc-2; lane 3: hep-hcc-3; lane 4: hep-hcc-4; lane 5: hep-hcc-5; lane 6: hep-hcc-6; lane 7: HLE; lane 8: Hep3B; P: positive control (testis as a positive control). RT–PCR for G3PDH was used to monitor the quality of the RNA sample.