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. 2005 Aug 9;93(5):565–570. doi: 10.1038/sj.bjc.6602741

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Copyright © 2005 Cancer Research UK

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In the study of minimal residual disease in DTC, two thyrocyte-depleted individuals – suffering from medullary thyroid carcinoma and treated with thyroidectomy as well as with radio-iodine therapy – served as negative controls (MTC patients 1 and 2). RT–PCR amplification for Tg (lanes 1+3, product size: 405 bp) and PBGD (lanes 2+4, product size: 253 bp) transcripts was carried out following harvest of nucleated peripheral blood cells by density gradient centrifugation (M=100 bp DNA ladder). A notable expression of the supposedly thyrocyte-specific Tg is shown (PAGE, silver staining).