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. 2005 Sep 6;93(7):774–780. doi: 10.1038/sj.bjc.6602780

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Copyright © 2005 Cancer Research UK

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(A) Proteasome proteolytic activity, as measured by the ‘chymotrypsin-like’ enzyme activity, in pectoral muscle of mice bearing the MAC16 tumour and with different extents of weight loss. (B) Expression of C5 mRNA and (C) expression of C2 mRNA in pectoral muscle from cachectic mice bearing the MAC16 tumour. (D) Expression of 20S proteasome α-subunits, determined by immunoblotting, in soluble extracts of pectoral muscle from mice bearing the MAC16 tumour and different extents of weight loss. (E) Densitometric analysis of band intensities in (D). Differences from animals without weight loss are expressed as a, P<0.05, b, P<0.01 and c <0.001. (F) Relationship between weight of pectoral muscle and percentage weight loss.