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. 2005 Sep 6;93(7):774–780. doi: 10.1038/sj.bjc.6602780

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Copyright © 2005 Cancer Research UK

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(A) Expression of PIF in urinary extracts of mice bearing the MAC16 tumour. Urine (2 ml) was treated with ammonium sulphate (80% w v⁻¹) and stirred overnight at 4°C. The precipitated protein was recovered by centrifugation at 3000 g for 30 min, dialysed against water using an Amicon filtration cell and 15 μg was used for immunoblotting using anti-PIF monoclonal antibody. (B) Densitometric analysis of the band intensities in (A) reported in arbitary units.