Table 1. Sequences of primers used in RT–PCR and real-time RT–PCR analysis.

	Primers	TaqMan probe
Conventional RT–PCR
NKD1 (NM_033119)	F, 5′-CGCCGGGATAGAAAACTACA-3′
	R, 5′-CTGGAGCTCTGAGACCTTGG-3′
FZD3 (NM_017412)	F, 5′-ACATCCACCCATGCTTCTTC-3′
	R, 5′-TCGTGACTGTTCATTGAGCC-3′
FZD4 (NM_012193)	F, 5′-CCAACATGGCTGTTGAAATG-3′
	R, 5′-GTCTCACTGCCTTTTCCAGG-3′
FZD6 (NM_003506)	F, 5′-ATGAGAGAGGTGAAAGCGGA-3′
	R, 5′-TTGGTTCTGAAGAACTGGGG-3′
FZD10 (NM_007197)	F, 5′-CCTCCAAGACTCTGCAGTCC-3′
	R, 5′-GACTGGGCAGGGATCTCATA-3′
Real-time PCR
NKD1 (NM_033119)	F, 5′-TCGCCGGGATAGAAAACTACA-3′	P, 5′-CCAATTTGGGCCTGGCTCCCC-3′
	R, 5′-CAGTTCTGACTTCTGGGCCAC-3′
FZD3 (NM_017412)	F, 5′-CATGGAGATGTTTGGTGTTCCTT-3′	P, 5′-TCTGGGAACCTACTGCATTCCATATCTTCAGG-3′
	R, 5′-AAGTCGAGGATATGGCTCATCAC-3′
KRT8 (NM_002273)	F, 5′-GATCGCCACCTACAGGAAGCT-3′	P, 5′-CCGGCTCTCCTCGCCCTCCA-3′
	R, 5′-ACTCATGTTCTGCATCCCAGACT-3′
FZD6 (NM_003506)	F, 5′-ATCTGATGGGTCATTATGACCAGAGT-3′
	R, 5′- TTTGCGAGAGGAAGAAAATGCT-3′