Figure 3. Differentiation of PC12 cells with nerve growth factor (NGF) increases the association of PRV gE and gC with DRMs, but gH remains soluble.

PC12 cells were differentiated in low serum conditions in the presence of NGF for 12 days. Cells were infected with PRV Becker for 12 hours, and then lysed with cold 1% Triton X-100. Lysates were separated on a discontinuous Optiprep™ density gradient, and analyzed by SDS-PAGE. Western blotting was performed using biotinylated cholera toxin B subunit (for GM1) and antibodies to PRV Us9, gB, gE, gC, gH and transferrin receptor (TfR). The preprocessed form of gB (*) is labeled along with the 69 kDa and 58 kDa (−) subunits.