Figure 4. The raft association of Us9 is not dependent on the presence of gE or the Us9 acidic cluster motif.

Non-differentiated PC12 Cells were infected with PRV 99 (A) or PRV 162 (B) for 12 hours. Cells were subsequently lysed with cold 1% Triton X-100, and separated on a discontinuous Optiprep™ density gradient. Samples were subjected to SDS-PAGE, and the presence of GM1, Us9, gE, and gH was assessed by Western blot analysis.