Skip to main content
. Author manuscript; available in PMC: 2009 Mar 14.
Published in final edited form as: Mol Cell. 2008 Mar 14;29(5):541–551. doi: 10.1016/j.molcel.2007.12.023

Figure 2. Increased UPR in tumors arising in TSC2+/− mice.

Figure 2

(A) Hematoxylin & Eosin staining of the sections from the liver and (B) kidneys of Tsc2+/− mice at 12 months of age illustrating the presence of hemangiomas and adenomas, respectively. (C) Immunofluoresence staining of phospho-S6 (Ser235/236), phospho-IRE-1, phospho-PERK (Thr980) and phospho-eIF2α (ser51) in the liver hemangiomas of Tsc2+/− mice. (D) Immunofluoresence staining for phospho-S6 and GRP78 in the same cystic adenoma shown in panel B. (E) Detection of phospho-S6K1 (Thr389), total S6K1, phospho-PERK (Thr980), phospho-eIF2α (ser51) and total eIF2α levels by immunoblotting extracts from either adjacent normal kidney tissue (N) or kidney adenomas (T) removed from 20-months old Tsc2+/− mice treated with either rapamycin (10 mg/kg) or vehicle for 2 consecutive days. Mice were sacrificed and extracts were prepared 12 hours after the second dose. Representative samples are shown.