Figure 2.

Analysis of integrin β1 knockdown cell lines. (A) Flow cytometry analysis of integrin β1 expression. Control and integrin β1 knockdown clones were stained using P5D2 anti-integrin β1 antibody or no antibody and 10 000 cells analysed by flow cytometry. (B) Control and integrin β1 knockdown cells (clone 20 shown) were stained with P5D2 antiintegrin β1 antibody (green) and phalloidin to visualise F-actin (red). (C) Control and integrin β1 knockdown cells (clone 4 shown) were stained with phalloidin to visualise F-actin (left) and vinculin (middle) and phospho-Y118 paxillin (right) to visualise focal adhesions.