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. 2004 Dec 7;92(1):30–35. doi: 10.1038/sj.bjc.6602271

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Copyright © 2005 Cancer Research UK

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LOH analysis of p53 gene by DHPLC. The analysis was performed at 50°C and flow rate 0.9 ml min⁻¹ in a gradient of acetonitrile in 0.1 M triethylammonium acetate: the gradient started at 8.75%, increased in 3.5 min to 13.75%, then increased in 7 min to 16.25%, was constant for 1 min, then increased in 1 min to 75%, was constant for 1 min (wash), then decreased in 1 min to 8.75% and finally was constant for 1 min (equilibration).