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. 2005 Jan 11;92(2):405–412. doi: 10.1038/sj.bjc.6602319

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Copyright © 2005 Cancer Research UK

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Titration of PNA concentrations for complete suppression of amplification of 1 μg of wild-type DNA (WT DNA; human placenta DNA) in the presence of 100 pg of mutated DNA (10 000 : 1) extracted from cells of the colon carcinoma cell line SW 480, which bears the codon 12 mutation of the Ki-ras proto-oncogene (GGT to GTT; glycine to valine) homozygous. After rapid cycle amplification of the DNA in the presence of the valine mutation-specific hybridisation probes, the melting curves were analysed by the LightCycler software (version 3.5). Temperature transition rate was 0.3°C s⁻¹. 1: non template control (NTC); 2: 1 μg wild-type (WT) DNA without PNA; 3: 100 pg Val DNA without PNA; 4: 1 μg WT DNA/100 pg Val DNA without PNA; 5–8: 1 μg WT DNA/100 pg Val DNA each with raising concentrations of PNA as indicated. PCR experiments were performed twice.