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. 2005 Mar 1;92(5):882–887. doi: 10.1038/sj.bjc.6602362

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Copyright © 2005 Cancer Research UK

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Immunohistochemistry on sections of Ad.C28-sCE2-transduced Colo205 spheroids. Colo205 spheroids were transduced with 1 × 10⁷ PFU Ad.C28-sCE2 and harvested at day 1, 4 and 5 after transduction. Sections of these spheroids were made and stained for myc to detect C28-sCE2. (A) At day 1 after Ad.C28-sCE2 transduction, no positive staining can be detected. At days 4 and 5, several spots along the rim of the spheroid are positively stained. (B) A higher magnification of the fusion protein staining at day 5 after transduction is shown. Cells with clear staining of membranes only (arrows) represent untransduced neighbouring cells with bound C28-sCE2.