Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Apr 20;92(8):1430–1441. doi: 10.1038/sj.bjc.6602487

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005 Cancer Research UK

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Characterization and specificity of HGS-ETR1 antibody. (A) Direct-binding ELISA data show the binding of indicated concentrations of HGS-ETR1 to the TRAIL-R1 extracellular domain Fc-protein (filled circles) or other TRAIL-R extracellular domain Fc-proteins (open symbols) immobilised on 96-well plates. Binding of HGS-ETR1 to TRAIL-receptors was detected using anti-human Fab-peroxidase conjugate. (B) Binding of HGS-ETR1 to SW480 cells analysed by flow cytometry. The top panel shows the binding of isotype control monoclonal antibody (IC mAb, dotted line) and HGS-ETR1 (shaded peak). The bottom panels show the binding of HGS-ETR1 alone (shaded peak) or binding of HGS-ETR1 in presence of 5 μg ml⁻¹ of the indicated soluble TRAIL-R Fc-protein (solid line). (C) For viability assays, SW480 cells were plated at 1 × 10⁴ cells well⁻¹ in a 96-well plate and cultured overnight. A 1 μg ml⁻¹ concentration of HGS-ETR1 or isotype control monoclonal antibody (IC mAb) was added to the cells in the absence or presence of the indicated soluble TRAIL-receptor proteins (sTRAIL-R1-4, sOPG, 5 μg ml⁻¹) and cell viability, measured as relative light units (RLU), was determined after 48 h. Data is the mean±s.d. of triplicate samples.