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. 2005 Apr 5;92(8):1421–1429. doi: 10.1038/sj.bjc.6602526

Figure 2.

Figure 2

Protective vaccination of BALB/c mice. Five animals per group were immunised on days 0 and 14 by s.c. injection of Tc-ErbB2/Th-HA liposomes (○) or Tc-ErbB2 liposomes (•) corresponding to 15 μg of coupled Tc-ErbB2 peptide per injection. Control animals received PBS (open diamonds). On day 20, mice were inoculated with Renca-lacZ/ErbB2 tumour cells by s.c. injection into each flank. Tumour growth was followed by caliper measurements and mean tumour volumes were calculated. (A) Tumour-free survival. Tumour growth was registered when palpable tumours had formed at one or both of the injected flanks. (B) Kinetics of tumour growth of the experiment shown in (A). As indicated by asterisks, two of the Tc-ErbB2 vaccinated animals had to be killed on day 57 due to tumour growth. From day 64 onwards, mean tumour volume in this group was calculated from the remaining three animals. Tumour-bearing mice were killed latest when tumour size reached 0.8 cm3, to avoid suffering of the animals.