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. 2005 Apr 5;92(8):1421–1429. doi: 10.1038/sj.bjc.6602526

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Copyright © 2005 Cancer Research UK

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Specificity of tumour rejection. Two groups of BALB/c mice were immunised on days 0 and 14 by s.c. injection of Tc-ErbB2/Th-HA liposomes corresponding to 15 μg of coupled Tc-ErbB2 peptide per injection. On day 20, one group of animals was inoculated with Renca-lacZ/ErbB2 tumour cells (open circles) by s.c. injection into each flank. The other group was injected with ErbB2-negative parental Renca-lacZ cells (filled diamonds). Tumour growth was followed by caliper measurements and mean tumour volumes were calculated. Tumour-bearing mice were killed latest when tumour size reached 0.8 cm³, to avoid suffering of the animals.