Table 1. IC50 against MX2 and etoposide, adriamycin, and vincristine with or without indomethacin, 5-Aza-2′-deoxycytidine (5AZ) treatment.
| MX2 (nM) | Etoposide (nM) | Adriamycin (nM) | Vincristine (nM) | Carboplatin (μM) | |
|---|---|---|---|---|---|
| K562/P | 30±4 | 10±4 | 20±3 | 2.0±2.1 | 20±5.1 |
| K562/P with indomethacin | 28±7 | 9±5 | 18±3 | 1.8±2.0 | ND |
| K562/P with 2 μM 5AZ | 28±5 | 8±5 | ND | ND | ND |
| K562/P with 10 μM 5AZ | 29±6 | 7±4 | 15.0±11.0 | 1.8±2.9 | 22±4.8 |
| K562/MX2 | 200±23* | 94±15* | 150±20* | 2.3±1.8 | 18±6.0 |
| K562/MX2 with indomethacin | 190±50* | 90±25* | 100±25* | 1.8±1.0 | ND |
| K562/MX2 with 2 μM 5AZ | 50±12a | 10±5a | ND | ND | ND |
| K562/MX2 with 10 μM 5AZ | 46±9a | 8±5a | 130±10.7 | 2.0±1.0 | 20.5±4.8 |
| K562/ADM | 28±9.3 | 8.2±3.0 | 250±50* | 20.2±8.1* | 21±6.8 |
| K562/ADM with indomethacin | ND | ND | 50±50** | 5±3** | ND |
| K562/ADM with 10 μM 5AZ | 30±8.8 | 9.3±2.5 | 100±20.8a | 5.0±2.8a | 20±5.0 |
ND=not done. IC50 was calculated from cytotoxicity against various drugs. Data were average±s.d. from five independent experiments.
*
P<0.05, cytotoxicity in K562/P vs K562/MX2, or K562/ADM cells.
**
P<0.05, cytotoxicity in K562 cells with vs without indomethacin treatment.
a
Cytotoxicity in K562 cells with vs without 5AZ treatment.