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. 2005 Apr 12;92(9):1773–1781. doi: 10.1038/sj.bjc.6602537

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Copyright © 2005 Cancer Research UK

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Activation ERK and JNK during treatment of MDA-MB-435 cells with garlic. (A) Western blot showing the amount of activated ERK1/2 (pERK) (antibodies against phospho-ERK1/2 (Thr202/Tyr204) were used) and total ERK (labelled ERK) at different times after addition of garlic. The amount of pERK after addition of PD98059 and DADS were also measured. (B) Western blot showing the amount of activated JNK (pJNK) and total JNK (labelled JNK) at different times after addition of garlic. Two main forms of JNK (p54 and p46) and a minor form below p46 were detected. The phospho-specific antibody recognised p46 and the form below.