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. 2005 Jan 25;92(3):539–546. doi: 10.1038/sj.bjc.6602365

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Copyright © 2005 Cancer Research UK

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Changes in the levels of membrane-bound PKCα following methacholine stimulation of intact rat parotid gland acinar cells, 10 days after in vivo (sham-) irradiation. Isolated parotid gland cells were treated with methacholine for 2 min at the indicated doses. (A) Protein kinase C alpha expression was determined by immunoblot analysis as described under ‘Materials and Methods’. 80- indicates molecular mass marker. Always 10 μg of protein used. (B) Diagram of at least three independent experiments. Significantly different from unirradiated situation: ^*P<0.05 (Student's t-test).