Figure 2.

DNA and amino acid sequences encoded by the exon 1 (A), exon 2 (B), and exon 3 (C) and flanking regions of the pbsA gene from R. violacea. DNA sequences underlined were confirmed by cDNA sequencing. The resulting RNA splice sites are marked by a †. Pentanucleotides characteristic of type II introns 5′ sequences are shaded. Nucleotides (or complementary sequences) in bold type correspond to oligonucleotides used as primers for PCR and RT-PCR. The 5′ end of mRNA is indicated by asterisks (∗) in A. Glutamic E*25 in B is encoded by GAA after the splicing event joining exons 2 and 3. Inverted repeat at the 3′ end of pbsA is indicated by the arrowed line in C.