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. Author manuscript; available in PMC: 2009 Apr 15.
Published in final edited form as: Dev Biol. 2008 Feb 15;316(2):441–455. doi: 10.1016/j.ydbio.2008.02.003

Figure 3.

Figure 3

Induction of Mia1 in embryonic tracheal epithelium. Intact distal lung tips, recombinations of E11.5 lung mesenchyme and lung epithelium, or recombinations of E11.5 lung mesenchyme and tracheal epithelium were prepared and cultured for 3 days. ISH of intact E11.5 distal lung tips (A,B) and lung mesenchyme + lung epithelium recombinants (C,D) demonstrates that Mia1 expression was sustained by lung epithelium in vitro. Recombination of lung mesenchyme with E11.5 tracheal epithelium, which was negative for Mia1 at the time of isolation (see Figure 1), results in the induction of Mia1 in the reprogrammed tracheal epithelium (E,F). All panels are at the same magnification; bar = 45 μm.