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. Author manuscript; available in PMC: 2009 Apr 15.
Published in final edited form as: Dev Biol. 2008 Feb 15;316(2):441–455. doi: 10.1016/j.ydbio.2008.02.003

Figure 8.

Figure 8

Gene expression in SP-C/MIA mice. Immunostaining of E18.5 lung sections for FLAG identifies epithelial cells misexpressing Mia1 (B), whereas single transgenic controls show no signal (A). Immunostaining for mature SP-B shows widespread expression in single transgenic (expressing only the tetO/MIA transgene) controls (C). SP-C/MIA mice, however, show greatly reduced SP-B staining (D), a finding that is confirmed by Western blot (G). Immunostaining for the type I cell marker T1α appears similar in both single transgenic and SP-C/MIA mice (E,F). All panels are at the same magnification. Comparison of the content of saturated phosphatidylcholine (SatPC) in the lungs of SP-C/MIA mice and controls demonstrates that the lungs of SP-C/MIA mice contain only 40% (*, p < 0.0001) of the SatPC found in single transgenic controls. Data are plotted as means ± SE (n = 6 for SP-C/MIA mice and 7 for controls).