Figure 2.

IFNAR1 expression in 2fTGH cells and derivatives. (A) Whole cell extracts (1 mg) were immunoprecipitated with the anti-IFNAR1 monoclonal EA12 antibody, fractionated on SDS/7% polyacrylamide gel and blotted with the anti-IFNAR1 monoclonal GB8 antibody. (B) Northern transfers of 10 μg of total RNA extracted from subconfluent untreated (−), IFN-α, or IFN-β treated cells (4 hr) were hybridized to an IFNAR1 cDNA specific probe. Comparable signals in each lane were obtained with a β-actin probe (not shown).