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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Mol Immunol. 2008 Mar 4;45(9):2667–2677. doi: 10.1016/j.molimm.2007.12.024

Fig 3. Promoter analysis of IE function.

Fig 3

a) Effects of C/EBPβ and the individual isoforms of IE on HT and DT reporters. Transfections were carried out in HeLa S3 cells as described in Fig 2 with the exception that Spi-1 expression vector was not used and the IE (1+2) vector (pEQ276) was substituted by vectors that express either IE1 (pEQ276) or IE2 (pEQ326), as indicated. Results are displayed as mean light units ± SEM of triplicate samples and are representative of at least three experiments. b) Schematic of mutations made at C/EBP sites in HT reporters used in (c). c) Gene reporter analysis of mutated CEBP sites on IL1B promoter. HeLa S3 cells were transiently transfected with mutant or wt IL1B promoter reporters and cotransfected with IE2 (pEQ326), C/EBPβ, and Spi-1 in six groups, as indicated. Results are displayed as mean light units ± SEM of triplicate samples and are representative of at least three experiments.