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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Mol Immunol. 2008 Mar 4;45(9):2667–2677. doi: 10.1016/j.molimm.2007.12.024

Fig 6. Localization and specificity of the IE2 291–364 peptide.

Fig 6

a) Cellular localization of GFP–IE2 291–364 fusion product. HeLa S3 cells were transiently transfected with IE2 291–364pEGFP. After 24 hr, plates were visualized by fluorescence microscopy. b) Functional analysis of the GFP–IE2 291–364 fusion product described in (a). HeLa S3 cells were transfected with the HT reporter and cotransfected with the following expression vectors: Group 1 = reporter only; group 2 = Spi-1 + C/EBPβ + GFP; group 3 = Spi-1 + C/EBPβ + GFP-IE2 291–364. 48 hours following transfection, cells were lifted and lysates analyzed by luminometry and displayed as the mean light units ± SEM. c) C/EBPβ expression from the pCDNA3.1 expression vector used in (b) was detected by Western blot ( 198, Santa Cruz). The lane numbers correspond to the transfection groups in (b).