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Complementation analysis of AST68. A wild-type strain, INVSc1, and the sulfate transporter-deficient mutant, YSD1, transformed with vectors pYES2 (control) and pYAT68 (harboring the AST68 coding region), were grown at 30°C on the synthetic minimal media containing galactose (20 g/liter) as a carbon source and 0.1 mM MgSO4 as a sulfur source as described in ref. 9.
