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. Author manuscript; available in PMC: 2009 Apr 4.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Jan 28;368(2):231–237. doi: 10.1016/j.bbrc.2008.01.085

FIG. 1.

FIG. 1

(A) Schematic for cloning of sCD14-EBD sequence (residues 20-171) in pPICZα A vector along with a His6-tag for expression in Pichia pastoris. Recombinant sCD14-EBD is secreted into the extracellular medium by the yeast α-factor secretion signal via cleavage at the Kex2 protease site. (B) Analysis of sCD14-EBD expression in Pichia pastoris by SDS-PAGE and Western blot of recombinant sCD14-EBD.