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. Author manuscript; available in PMC: 2009 Apr 4.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Jan 28;368(2):231–237. doi: 10.1016/j.bbrc.2008.01.085

FIG. 2.

FIG. 2

(A) Far-UV circular dichroism spectra of sCD14-EBD (blue line) and sCD14-EBD -LPS complex (red line). Both spectra are average of five scans. (B) Biological activity analysis of sCD14-EBD with endotoxin ligands. Responses of THP1-blue cells mediated by full-length wild-type sCD14 (rhCD14, 400 ng/mL) and purified sCD14-EBD (gCD14, 200 ng/mL) upon stimulation with three endotoxins, LPS (1 μg/mL), LTA (1 μg/mL) and MDP (200 ng/mL). After 24 h incubation, endotoxin stimulation was assessed by measuring the levels of NF-κB inducible reporter alkaline phosphatase (SEAP) using a SEAP detection medium with absorbance values at 630 nm. Control experiments in form of responses to rhCD14 and gCD14 alone are shown as well.