Figure 2.
Effect of oligonucleotides on DNA-PK activity. (A) Effect of S-dC6, S-dC12, S-dC24, S-dC36, S-dC48, and S-dC60 on DAN-PK activity. Whole cell extract prepared from LM217 was used. (B) Effect of S-dC36 on DNA-PK activity. Whole cell extract prepared from T98G was used. (C) Effect of S-dC36, S-dG36, S-dA36, S-dT36, and dC36 on DNA-PK activity. Whole cell extract prepared from LM217 was used. (D) Effect of S-dC36 and dC36 on DNA-PK activity. Purified DNA-PK was used. (E) Effect of HCl-treated S-dC36 and untreated S-dC36 on DNA-PK activity. Whole cell extract prepared from LM217 was used. Salt concentration of untreated S-dC36 was adjusted to that of HCl-treated S-dC36. DNA-PK activities are expressed as values relative to that of control, which is set to a value of 1. The data represents the means±s.d. (n=3).