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. 2002 Sep 9;87(6):673–680. doi: 10.1038/sj.bjc.6600518

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Copyright © 2002 Cancer Research UK

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Conditioned medium from DDAH I overexpressing cells induces endothelial cell migration in vitro. A human endothelial cell line SGHEC-7 was grown on beads in three dimensional fibrin gels and incubated with conditioned medium (cm) from confluent SGHEC-7, D27 or C6 cells. Quantitative analysis of invasive process formation was expressed as the average of the maximum length of process minus the radius of the bead per 40 MC beads. Results are means+s.e.m. of 40 individual measurements in three separate experiments (***P<0.001 compared to control, Student's t-test).