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. 2002 Sep 23;87(7):805–812. doi: 10.1038/sj.bjc.6600547

Figure 5.

Figure 5

Caspase 3 activation in SkBr3 cells treated with 2DG. SkBr3 cells were treated with 16 mM 2DG for the 4 or 6 h. Cells were harvested and caspase 3 activity measured using a spectrophotometric assay according to the manufacturer's instructions. The caspase 3 inhibitor DEVD-FMK was added 30 min prior to the addition of substrate in cells which had been incubated with 16 mM 2DG for 6 h. Each point represents the average of triplicate cultures. Control cells were incubated for 6 h in the presence of media only.