Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2002 Oct 21;87(9):1000–1005. doi: 10.1038/sj.bjc.6600589

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2002 Cancer Research UK

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Time course of the phosphorylation of c-Raf1 and MAPK in pK568MRSV transfected adrenocortical cells after treatment with IPTG. Crude protein extract from transfected cells was electrophoresised and transfered to PVDF as described in Materials and Methods. And then, it was immunobloted with monoclonal antibodies specific to phosphorylated c-Raf1 (p-Raf-1), phosphorylated MAPK and control c-Raf-1 and MAPK, respectively. The amounts of phosphorylated c-Raf-1 (p-Raf-1) and phosphorylated MAPK; and control MAPK and c-Raf-1 were detected at 0, 1, 6, 12, 24, 36 and 48 h after IPTG induction. The amounts of phosphorylated c-Raf-1 and MAPK were increased with the elongation of IPTG treatment. Plus (+) indicates when present; minus (−) indicates when absent.