Figure 1.

Immunochemical detection of protein carbonyls in mitochondrial membranes from flight muscles of houseflies. DNPH-treated proteins (10 μg) were electrophoresed on SDS/PAGE (10% resolving gel) under reducing conditions and transferred to Immobilon-P membrane. Oxidized proteins were detected immunochemically as described in the text. Lane 1, protein standard markers; lane 2, proteins without DNPH treatment; lanes 3–5, 5-, 10-, and 15-day-old fly mitochondrial proteins, respectively, treated with DNPH. A 33-kDa protein in the membranes exhibited a strong immunoreaction for the carbonyl groups.