Figure 3.

Immunochemical estimation of the carbonyl content of the 33-kDa protein in housefly at different ages. Presented values are mean ± SD of relative densitometric determinations in four independent experiments that used flies hatched on different dates. Immunostain intensity of the protein from 5-day-old flies was assigned an arbitrary value of 100; the ratio of 10- or 15-day-old flies and crawlers to the 5-day-old flies is reported. The increase in ANT carbonyl content between each age group was statistically significant.