Figure 5.

Effects of hyperoxia on ANT carbonyl formation and ANT activity. (A) Immunochemical estimation of ANT carbonyls in houseflies exposed to 100% ambient oxygen. Shown are relative densitometric values expressed as mean ± SD of four independent experiments. Immunostain intensity of the control was assigned an arbitrary value of 100, the ratio of oxygen-treated to control is reported. Flies that were 9 days old were exposed to 100% oxygen for indicated periods. (∗, P < 0.05 vs. 0 h; ∗∗, P < 0.01 vs. 48 h.) The changes between 0 and 24 h and between 24 and 48 h were not statistically significant. (B) Effect of 100% oxygen on the exchange activity of mitochondrial ANT. Shown are the mean ± SD of four independent determinations. (∗, P < 0.001 vs. control; ∗∗, P < 0.005 vs. 48 h.) The changes between each group of the control (no oxygen exposure) and between 24 and 48 h of oxygen exposure were not statistically significant.