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. 1998 Oct 27;95(22):12896–12901. doi: 10.1073/pnas.95.22.12896

Figure 7.

Figure 7

Immunochemical detection of HNE adducts in ANT. HNE detection was performed as described (23). Rabbit anti-HNE antibody (IgG) was diluted to 1:10,000 in Tris-buffered saline containing 0.1% Tween-20 with overnight exposure of the blot at 4°C. Goat anti-rabbit IgG conjugated with horseradish peroxidase was diluted 1:50,000 in Tris-buffered saline containing 0.1% Tween-20 with 3 h incubation at room temperature. (A) Age-associated changes in HNE level in ANT. (B) Comparison of HNE adducts in ANT between 15-day-old flies (lane 1) and crawlers (lane 2).