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. Author manuscript; available in PMC: 2008 May 1.
Published in final edited form as: Osteoarthritis Cartilage. 2007 Aug 13;16(2):185–194. doi: 10.1016/j.joca.2007.06.008

Figure 6. Fluorescent immunomicroscopy of IGFBP-3 and DAPI Stained Nuclei in Young Human Cartilage.

Figure 6

Panels A and C show the orange fluorescent stain for IGFBP-3 detected with Upstate anti-IGFBP-3 (1:250 dilution) and a CY-2 conjugated goat anti-rabbit secondary antibody (Jackson Immunoreasearch). Panels B and D show adjacent sections stained with DAPI to reveal the nuclei. Panel E shows an overlay of panels C & D demonstrating co-localization of IGFBP-3 and DAPI stain.