Skip to main content
. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Dev Biol. 2008 Jan 3;315(1):217–231. doi: 10.1016/j.ydbio.2007.12.039

Fig. 7. Increased apoptotic cell death of the lens fibers in the RhoGDIα transgenic eyes.

Fig. 7

P1 WT and Tg lens cryosections were examined for apoptotic cell death by detecting the digoxigenin-nucleotides using a fluorescein-tagged anti-digoxigenin antibody. These same lens sections were also co-stained with propidium iodide to localize nuclei. While WT lens sections (A) revealed no TUNEL positive cells in the fiber cells, degenerating lens fibers of Tg lens showed increased TUNEL positive immunostaining in the central (B) and posterior (C) regions (indicated with arrows). Epithelium from both WT and Tg lenses showed sporadic TUNEL positive cells but with no significant difference between the two types of lenses. A representative image is shown based on multiple analyses.