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. Author manuscript; available in PMC: 2009 Apr 15.
Published in final edited form as: Biochem Pharmacol. 2008 Jan 20;75(8):1610–1615. doi: 10.1016/j.bcp.2008.01.006

Figure 2. Time course of AZT phosphorylation in cultured cells.

Figure 2

Flasks of cells (U-937, Raji, and H9c2) were incubated for up to 48 hours with the addition of 1 μM [methyl-3H]-AZT (2200 DPM/pmol) to the growth media. Samples were prepared as described in Materials and Methods and the DPM s obtained converted to pmoles by dividing by the specific radioactivity of the AZT pool for that experiment. Data represent the mean ± SEM of three to four trials. AZTMP (left) and the sums of AZTDP and AZTTP (right) from the cellular extracts are shown as pmol per 106 cells versus hours of incubation.